biology If an end point of flame was found with 400uL of 95% standard diluted with 600uL of dH20, then what is the concentration of an unknown fuel if 550uL of the unknown fuel is mixed with 450uL of dH20 to find its end point of flame

What mistake did john needham make that caused him to conclude that spontaneous generation for microorganisms occurred? a. he re-contaminated his boiled broth solutions. b. he destroyed the vital force in the solutions. c. he did not boil his broth solutions, only warmed them. d. he failed to seal his flasks of boiled broth. e. he allowed his assistant to conduct the experiment which he did not monitor closely.