Your research team has discovered a new bacterial protein. You have cloned it, expressed it (produced it), and purified it. How can you verify that the protein you have purified is indeed the protein you are interested in (to make sure there has not been a mistake during the purification procedure)?
After you have confirmed the purified protein is the protein of interest, now, you like to study how this protein moves around in a bacterial cell. How do you go about this task, that is, how will you be able to monitor the protein’s location in a living cell for an extended period of time, e. g. several hours?