Suppose we were successful in identifying 12 mutants using a screen for identifying conditional mutants of S. cerevisiae in which the secretory system was defective. Part A Describe the crosses you would perform to determine the number of different genes represented by the 12 mutations. Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Terms can be used once, more than once, or not at all.
a. dominant
b. permissive
c. mutant
d. codominant
e. wild type
f. restrictive
g. recessive
Each mutant would be mated to ___ and to every other to create diploid strains. The diploids would be assayed for growth at and restrictive temperature. Diploids formed by mating a mutant to a that can grow at temperatures identify the mutation as . Only ___ mutations can be studied using complementation analysis. Diploids formed by mating two ___ mutants identify mutations in the same gene if the diploid cannot grow at ___temperature (non-complementation), and they identify mutations in different genes if the diploids can grow at temperature (complementation).
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Suppose we were successful in identifying 12 mutants using a screen for identifying conditional muta...
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