The arrows between the circulatory system and respiratory system show . The arrow going from the respiratory system to the circulatory system shows .

The arrow going from the circulatory system back to the respiratory system shows .

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.

emerging idea only occurs as a raw idea that could appear out of nowhere. meanwhile, to reach scientific consensus , that idea must be structured and tested over and over again through various experiments. the scientific consensus transforms the raw idea into idea that is more reliable through the process of scientific elimination.

answer-

the statement that f(x) = |x+a| + b has exactly one x-intercept is sometimes correct.

solution-

it solely depends on b, whether the function will have one or two or zero x intercept.

this plot of the given function, f(x) = |x+a| + b will be the basic absolute value graph i.e v shape, with vertex translated to (-a, b), instead of origin.

1-   if b is zero, the graph will have exactly one x-intercept, at x= -a

2- if b is positive, the whole graph will be above the x-axis, hence it will have no x-intercepts.

3- if b is negative, the graph will be below the x-axis, hence it will have two x-intercepts.

∴ the statement is sometimes true.

the oldest known insects are believed to live around 400 millions ago as suggested by the fossil records.   some plants having woody stems also evolved during this time.

after the evolution of insects the first four legged species or the tetrapods evolved in shallow freshwater habitats from the intermediate species approximately 397 million years ago. the tetrapods went on conquering the land giving rise to amphibians, reptiles, mammals and birds.

the major split in tetrapods first occurred 340 million years ago branching off from amphibians.

363 million years ago when the carboniferous period began the insects started roaming on land and taking flights, following which the four limbed tetrapods adapted in the terrestrial habitat.