Answer parts A, B, C, D Photo Attached

a). a microRNA (miRNA) is a small non-coding RNA involved in transcriptional and post transcriptional regulation of gene expression. Given the mRNA molecule below, suggest an example of a complementary 10 nucleotide miRNA. circle or highlight the sequence on the mRNA template to which your miRNA will bind, give the complementary 10 nucleotide sequence and clearly label the orientation of the miRNA molecule.
mRNA region: 5’-GUAGUACUGAUGGUCCAGUACGUGCUAGGUCG CUAGUAGUUCGAUGCAGUAUUAU-3’

b). what is the consequence of this miRNA binding to the mRNA above?

c). what happens when the miRNA is not completely complementary to the mRNA it is attached to?

d). what is one theory explaining the evolution of the RNAi pathway? what important research function has RNAi served since its discovery?

Ms. w, a 21-year-old woman, came into a clinic after suffering a deep laceration on her foot while walking barefoot around her yard. the wound was cleaned, sutured, and bandaged, and she was released to return home after receiving tetanus antitoxoid. within 72 hours, the wound area was red and swollen, the suture line was dark in color, and it was accompanied by severe throbbing pain. ms. w had a high fever, her heart felt like it was racing, and she was finding it hard to concentraten even on simple tasks. she returned to the clinic and was immediately taken to the hospital. following lab tests, a diagnosis of acute necrotizing fasciitis was made. discussion questions 1. explain why ms. w. received a tetanus antitoxoid before leaving the hospital. (see chapters 3 and 4, infection and passive immunity.) 2. explain how acute necrotizing fasciitis developed in this case and the pathophysiology involved. (see acute necrotizing fasciitis.) 3. what is the potential outcome for ms. w if antibiotic drugs do not reduce the infection quickly?