Biology, 01.05.2021 01:30, herchellann302
The genome of a newly discovered bacteriophage is a linear DNA molecule 10,500 nucleotide pairs in length. One sample of this DNA has been incubated with restriction enzyme X and another sample with restriction enzyme Y. The lengths (in thousands of base pairs) of the restriction fragments produced by the two enzymes have been determined by gel electrophoresis to be as follows:
Enzyme X: Fragment X1 = 4.5; X2 = 3.6; X3 = 2.4
Enzyme Y: Fragment Y1 = 5.2; Y2 = 3.8; Y3 = 1.5
Next, the fragments from the enzyme X reaction are isolated and treated with enzyme Y, and the fragments from the enzyme Y reaction are treated with enzyme X. The results are as follows:
X fragments treated with Y: X — 1 → 4.5 (unchanged)
X − 2→2.1 + 1.5
X − 3→1.7 + 0.7
Y fragments treated with X:Y − 1→4.5 + 0.7
Y – 2→2.1 + 1.7
Y − 3→1.5 (unchanged)
Required:
Draw a restriction map of the phage DNA, indicating the positions of all enzyme X and enzyme Y restriction sites and the lengths of DNA between them.
Answers: 2
Biology, 21.06.2019 22:00, amberreid028
Which is the correct first step in finding the area of the base of a cylinder with a volume of 26x cubic meters and a height of 6.5 meters? v=bh 6.5=b(26x) v=bh v=26pi+(6.5) v=bh v=26pi(6.5) v=bh 26pi=b(6.5)
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Biology, 22.06.2019 05:30, angelesramos112
One important development during the 3rd trimester that will insulate the infant against changes in temperature is a. the deposition of fat under the skin b. the closing of the septum between c. the atria of the heart d. the beginnings of lung functioning e. the beginnings of light and sound sensing
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Biology, 22.06.2019 12:00, princessmoon
Which of the following is not associated with invasive species?
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The genome of a newly discovered bacteriophage is a linear DNA molecule 10,500 nucleotide pairs in l...
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