Suppose you are a scientist trying to people who cannot produce an enzyme needed for proper digestion. how could you use genetic engineering techniques to make transformed bacteria that produce the enzyme?

By using genetic engineering techniques to transform bacteria that produce the enzyme I will extract the DNA from the cells of different people who can make the enzyme digestion. Then will cut the DNA with a restriction enzyme, and use gel electrophoresis and a DNA probe to locate the gene. By using the polymerase chain reaction I will copy the gene. Thereafter, I will choose a plasmid that has an antibiotic resistance genetic marker, and cut the plasmid with the same restriction enzyme used to cut out the human gene.
Later I will insert the copies of the human gene into the plasmids and allow the bacterial cells to take in the plasmids. Later, I will allow the bacteria to grow in a culture containing the antibiotic. And hence, these bacteria will make the digestion enzyme.