You are tasked with characterizing responses to Epidermal Growth Factor (EGF) treatment of HeLa Cells. You grow HeLa cells then replace the culture media with media containing EGF at various concentrations, then harvest samples for Western blot analysis using an anti-phospho-MAPK antibody as a readout of receptor tyrosine kinase activity. As expected, your control (EGF treated cells) show a strong phopho-MAPK signal on your Western blot. What do you hypothesize will happen if you pre-treat HeLa cells with siRNAs (RNAi) against Ras prior to adding EGF and performing Western blot analysis with anti-phospho-MAPK
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You are tasked with characterizing responses to Epidermal Growth Factor (EGF) treatment of HeLa Cell...
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