You are studying a eukaryotic gene that codes for the alpha-subunit in hemoglobin. what is the most likely result if base substitutions occur in the first nucleotide at the 5' end of an intron and also in the last nucleotide at the 3' end?

Below are the choices:

There will be no change in the polypeptide coded by this gene because introns are not expressed.

A change in reading frame will alter all amino acids in the polypeptide past the point of the first substitution.

A change in reading frame will alter all amino acids in the polypeptide past the point of the second substitution.

This intron will not be removed during pre-mRNA splicing because the spliceosome will not recognize the intron-exon junctions.

Each base substitution is likely to alter only one amino acid in the final polypeptide chain.

The answer is This intron will not be removed during pre-mRNA splicing because the spliceosome will not recognize the intron-exon junctions.

The main role of dna in the  cell is the long-term storage of information. it is often compared to a blueprint, since it contains the instructions to construct other components of the  cell, such as  proteins  and rna molecules.the main function of rna is to carry information of amino acid sequence from the genes to where  proteins  are assembled on  ribosomes  in the  cytoplasm. mark brainliest, my work is good.