25. E1, E2, ubiquitin, ATP, and both native and heat-inactivated (denoted by an asterisk, *) GST-ARD1 (1-574), GST, and GST-cytohesin-1 (C-1) were mixed and run on a gel. A western blot was performed with an anti-ubiquitin antibody. a. Based on these results, what three proteins are required for ubiquitination

Explanation:

From the given information; It is pertinent to understand that hIgh mass stepping stool comparing to polyubiquitination of protein is gotten when each and every constituent of the chemical enzymes are added to the in-vitro framework. In view of these outcomes, it is obvious that E1, E2, and E3 ligase are needed for ubiquitination.