The 3-dimensional structure of RNA polymerase, the enzyme that catalyzes formation of the phosphodiester bond during RNA synthesis (i. e. transcription), was determined using X-ray crystallography. What is the correct chronological order of steps
describing how this was possible using this technique? 3 pts
#1: Aim an X-ray beam through the crystal, atoms that comprise your protein that is crystallized diffract the X-rays.
#2: Determine the pattern of x-rays (X-ray diffraction pattern) using a digital detector.
#3: Using data from the x-ray diffraction pattern and amino acid sequence, build a 3-D model of the protein using computer
software.
#4: Obtain a pure sample of protein that is very concentrated and therefore forms a crystal.
A/ #1 ->#2 -> #3 -> #4
B/ #3 -> #4 -> # 1 -> #2
C/ #4 ->#3 ->#1 -> #2
D/ #4 -> #1 -> #2 -> #3

answer; /// i believe the name your looking for is called (plaque)or check good luck

step 1: initiation

initiation is the beginning of transcription. it occurs when the enzyme rna polymerase binds to a region of a gene called the promoter. this signals the dna to unwind so the enzyme can ‘‘read’’ the bases in one of the dna strands. the enzyme is now ready to make a strand of mrna with a complementary sequence of bases.

step 2: elongation

elongation is the addition of nucleotides to the mrna strand. rna polymerase reads the unwound dna strand and builds the mrna molecule, using complementary base pairs. there is a brief time during this process when the newly formed rna is bound to the unwound dna. during this process, an adenine (a) in the dna binds to an uracil (u) in the rna.

step 3: termination

termination is the ending of transcription, and occurs when rna polymerase crosses a stop (termination) sequence in the gene. the mrna strand is complete, and it detaches from dna.

silty loam

hope this