You will calculate the concentrations of the components required for a chromium release
cytotoxicity assay. Target cells are labelled with radioactive chromium and incubated with CD8
effector cells for 6 hours. The release of the radioactivity can be measured as counts per minute
(cpm) and the amount of target cell killing calculated.
Your assay has radioactively labelled tumour cell targets and you will add your killer cells, called
effectors, to the targets.
The effector cells will be added to the target cells in increasing numbers to give effector:target
ratios of 40:1, 20:1, 10:1 and 5:1.
Part 1
a) You have radioactively labelled target cells at a concentration of 2 x 10 per ml.
You need 1 x
10 cells in each well, which will be constant, for your assay.
Volume of target cells required()ul
Answers: 1
Biology, 21.06.2019 22:00, tylerjoshonti
For a to be healthy, it has to have lots of different kinds of plants and animals. all of the different types of plants and animals in an ecosystem. name four types of ecosystems from those listed in the video. b) c) d) most living things live here. all living things depend on how an ecosystem like a game of sticks? why should we build new buildings on existing land? name four extinct species from those listed in the video. b) c) d) the best way to wipe out a species is to the largest ecosystem in the world is this percent of the world is covered by water. how many of all species in the world live in the ocean? list five things you can do to increase biodiversity. b) c) d) e) how many species are we losing per hour?
Answers: 3
You will calculate the concentrations of the components required for a chromium release
cytotoxicit...
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