What might happen if the order of expression of the
highlighted Hox gene was
disrupted, moved, or changed by mutation?

Similarly, mutations in the Hox genes can result in body parts and limbs in the wrong place along the body. Like a play director, the Hox genes do not act in the play or participate in limb formation themselves. The protein product of each Hox gene is a transcription factor.

This is giving me a headache i have asked others and they don’t know it either

Active site is where they touch.