You wish to express a particular peptide in E. coli using a high copy number plasmid. You have the amino acid sequence for the peptide. a. Explain the experimental process for generating and selecting the genetically engineered E coli using restriction enzymes, ligase, E. coli, plasmid with neomycin resistance, and known amino acid sequence. b. What control elements would you place on the plasmid to regulate expression and to prevent read-through

First idea: suddenly, women were leaving their homes to cycle and socialize on country roads and city streets. —wheels of change, sue macy second idea: it was not a stretch for some cyclists to see the possibility of a larger role for women in the world. —wheels of change, sue macy what type of graphic organizer would best represent the connection between these two ideas? 1) a t-chart that separates ideas into two different categories 2) a chronology that shows 3) a sequence of several events a cause-and-effect graphic that shows how one idea led to another 4)a problem-solution graphic that presents a problem and a solution to the problem