You have just trypsinized your monolayer cells growing in a T25 flask. Once detached you resuspended all of your cells in media. If you were to subculture some of your cells into a new T25 cells by taking 1.5 mL of cell suspension from a total of 3 mL and transferring it into a new T25 flask containing 8 mL of fresh media, your final dilution would be:
Answers: 1
mL
mL
mL
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You have just trypsinized your monolayer cells growing in a T25 flask. Once detached you resuspended...
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