You design primers for colony PCR with one primer binding to your gRNA insert (20 bp), and 1 primer binding to a region of your plasmid ~430 base pairs away. Your plasmid vector is pML104, which is 11,240 base pairs. In colony PCR, a positive result (indicating a colony likely carries a pML104 plasmid with the gRNA inserted) would be a PCR product that is:

The third stage of cellular respiration is (2 points)

1. an enzyme is considered a(n) because it speeds up a chemical reaction without being used up.2. in a catalyzed reaction, a reactant is often called a(n) . an enzyme is specific because the shape of its matches only particular reactants.4. an enzyme speeds up reactions by lowering the . the between an active site and its substrate often strains bonds and the reaction proceed.6. a( , which is often a vitamin, binds to an enzyme and plays a role in catalysis.7. high temperatures or changes in ph can an enzyme, causing it to lose its shape and biological activity.

Select all the correct answers. which statements fail to meet the requirements of a scientific claim? a). in the final 100 meters of a 10,000-meter race, an athlete's speed is more strongly related to anaerobic efficiency than to aerobic efficiency. b). flowers that reflect the most ultraviolet light are more likely to attract bees and other pollinators than flowers that reflect less ultraviolet light. c). the evidence supporting newton's laws of motion was accurate in newton's time, but the universe operates differently today. d). even though evidence indicates that the solar system's planets follow elliptical paths, copernicus's model involving circular orbits is true.