Biology
Biology, 19.01.2021 19:20, Tyrant4life

Consider our yeast CRISPR-Cas9 experiment: yeast are transformed with the PML 104-9RNA1 plasmid + HDR1 template to edit the ADE2 gene. Enter your answers below. a. In yeast, what is the template from which the gRNA is transcribed?
b. The HDR fragment must enter the of the yeast cell to be used for homology directed repair.
c. The Cas9 gene is transcribed from the as template, the transcript is transported from the to the of the cell, and then the protein is translated.
d. After translation of Cas9, what happens? Explain in ONE sentence.

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Consider our yeast CRISPR-Cas9 experiment: yeast are transformed with the PML 104-9RNA1 plasmid + HD...

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