Describe an experiment you would perform to test a proposed chloroplast-targeting signal sequence. Be specific about the type of cell and the proteins you would use. Describe the results you would expect if the sequence is indeed a chloroplast-targeting signal.

I would fuse the gene encoding the protein carrying the putative chloroplast-targeting signal sequence with the gene encoding Green Fluorescence protein. The GFP-tagged target protein enable to study the expression of the putative chloroplast localization signal sequence and its final cellular localization

Explanation:

The Green Fluorescence protein (GFP) is a protein widely used in molecular biology to investigate the expression of specific genes and then track the movement of proteins in living cells. This protein also can be used as a cell marker and to identify protein-protein interactions. When GFP is excited to a given wavelength (excitation peak 395 nm), it emits green light (emission peak 509 nm), thereby the expression/localization of the GFP-target fusion constructs can be evidenced by using a fluorescence microscope.

tsd is temerature dependent sex determination.

tsd: it is a type of environmental sex determination in which the temperature experienced during embryonic/ larval development (ambient temperature for development) determine the sex of the offspring.

crocodile displays the tsd where the ambient temperature of the developing egg determines the individual's sex. for example in the american alligator's eggs, incubation at 33 ºc produces mostly males, while incubation at 30 ºc produces mostly females. so if the american alligator's egg will get the ambient temperature (33 degree celcius)during embryonic development from egg, the offspring will be male and same for the female sex determination.