series of experiments in which 32P-labeled inorganic phosphate were introduced to erythrocytes (red blood cells) undergoing glycolysis. Would you expect to see the labeled phosphate in any glycolytic intermediates

The labeled inorganic phosphate will be found in 1,3-bisphosphoglycerate

Explanation:

In the fifth reaction step of glycolysis, glyceraldehyde-3-phosphate is oxidized by the enzyme glyceraldehyde-3-phosphate dehydrogenase to 1,3-bisphosphoglycerate. Two molecules of NAD+ are reduced to form two NADH + 2H⁺. The energy released by the oxidation process is used to add an inorganic phosphate group to position 1 of two molecules of glyceraldehyde-3-phosphate to form two molecules of 1,3-biphosphoglycerate.

In the sixth step, the two molecules of 1,3-biphosphoglycerate are converted to 3-phosphoglycerate, with the high-energy phosphate group in position 1 added to ADP producing 2 ATP through substrate-level phosphorylation.

Since in the experiments,32-P-labelled inorganic phosphate were introduced to erythrocytes undergoing glycolysis, the inorganic phosphates would be expected to seen in the molecules of 1,3-biphosphoglycerate. However, since, the phosphate group is subsequently added to ADP to form ATP, it will no longer appear in other intermediates.

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