Why is it important to perform a restriction digest after cloning your gene of interest fragments into the TOPO vector?

Restriction enzymes (restriction endonucleases) are proteins that cut DNA at (or close ... To be able to clone a DNA insert into a cloning or expression vector, both have ... you could carry out the two digestions simultaneously (double digestion)

Explanation:

both anaerobic and aerobic respiration use glucose (or other carbohydrates), proteins and lipids to produce atp and nadh. cell uses those energy molecules, obtained from food and   breaks them down during the chemical reactions of respiration. in anaerobic respiration, nadh is regenerated to nad+ by enzymes lactate dehydrogenase (lactate is produced, e.g. in muscle cells) or by alcohol dehydrogenase where ethanol is produced (alcohol fermentation), while in aerobic respiration, electrons from nadh are deposited in the mitochondria to regenerate nad+ (electron transport chain. anaerobic respiration produces 2 atp per molecule of glucose compared to aerobic respiration where about 38 atp molecules are produced.

the most common energy conversion is from kinetic energy to potential energy, and then from potential energy to kinetic energy.

later on comes the heat energy, mechanical energy, and other energy conversions.

this energy conversion is so common that we can find it in our day to day lives as well.

- one of the most common energy conversions involves the changing of potential energy to kinetic energy or kinetic energy to potential energy