Your lab uses the annelid worm Lumbriculus variegatus as a model organism to study regeneration. Your studies would be helped by knowing which genes are present in this organism, but its genome has not been sequenced. A recent technique for gene determination is RNA-Seq, in which RNAs are directly isolated and sequenced. What is a disadvantage of using RNA-Seq for gene identification? a. RNA-Seq may miss genes transcribed in a very tissue- and temporal-limited fashion.
b. RNA-Seq relies on genes being homologous across species, but not all genes have homologs.
c. RNA-Seq works only in mammalian cells and therefore cannot be used with model organisms such as Lumbriculus or Drosophila.
d. RNA-Seq may selectively miss noncoding RNAs as compared to mRNAs.