why would gel filtration chromatography be the best method for purifying an antigen for injection into a mouse to generate monoclonal antibodies

The question is incomplete and the options are not given, from my searches I was able to get the completed part which are;

A. Antibodies can be used to detect the antigen on a western blot

B. Antigens can be centrifuged through a column and pellet at specific speeds C. Antigens can easily be purified by charge and size when coated with SDS D. Antigens will separate by size going through the column beads and their holes

E. Organelles containing the antigen will have a specific buoyancy and separate in a gradient.

The correct option is D.

Antigens will separate by size going through the column beads and their holes

Antibodies can be used to detect the antigen on a western blot

Explanation:

The best method for purifying an antigen for injection into a mouse to generate monoclonal antibodies by gel filtration chromatography is Antigens will separate by size going through the column beads and their holes because Gel filtration chromatography is a size exclusion chromatography that work on the principle that molecules are separated based on their big sizers or larger sizes. The columns are designed to remove substances that are smaller in sizes. The larger size antigen elute first.

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the nitrogen cycle provides nitrogen to the ecosystem from the atmosphere, ground and oceans. nitrogen is an important component of complex molecules such as amino acids and nucleotides, which lead to the creation of proteins and dna, the building blocks of all life. plants absorb nitrogen in the form of ammonium, nitrate ion and, on rare occasions, as amino acids. animals receive nitrogen necessary for biological processes from feeding on living or dead organic matter.