Primer design is a critical step for the detection of a particular sequence in a sample. Which of the following would be true of primer design?

The group of choices is as follow:

A. RNA polymerase are added to the PCR mix to design random primers  

B. If detecting viral infection by PCR, the primers must not be complimentary (match) to regions of human DNA  

C. Pairs of primers must be designed, one to start amplification with Taq polymerase, the other to start amplification with human polymerase  

D. Primers are optional if you want to amplify all DNA within a sample

The correct answer is - B. If detecting viral infection by PCR, the primers must be complementary to regions of human DNA.

Explanation:

Short, single-stranded segments of DNA sequences utilize in the real-time PCR or polymerase chain reaction technique are called primers. Primers used to hybridize the region of the segment needed to be amplified.

The human polymerase enzyme is not used in the PCR reaction and requires a 3'OH group to synthesize the DNA by amplification which is possible only in the presence of primers as they have a 3'OH.

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explanation:

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