A sample of unknown concentration is diluted by placing 0.2 mL into 9.8 mL of buffer and then 3 mL is placed into a standard 1x1 cm square cuvette. It is measured at 350 nm and the Abs is 0.3. The molar absorptivity is 12.3 L/mol*cm. What is the concentration of the undiluted sample (mol/L to 2 decimal places)?

The answer is "".

Explanation:

The molar absorptivity value is=  12.3    

path length of cell = 1  

Absorbance = 1.2

Using the beer's lambert law:

Claculating the concentration of the solution after dilution:

find out the dilution value before concentration :

The volume taken by the dilution:

The final volume after dilution:

Formula:

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