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Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pKa = 6.0) when the other has a valine (uncharged side chain).1. Which would be better for separation: gel electrophoresis or isoelectric focusing?A. Gel electrophoresis. B. Isoelectric focusing.2. What would you choose for the separation?A. At a pH of 6.0.B. At a pH above 6.0.C. At a pH below 6.0.D. At any pH value.
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