During genetic engineering, how do Restriction enzymes know what base pairs to act on? A. Ligase bonds it to the correct spot. B. They don’t, the scientist must target it manually. C. They bond to the Recognition site. D. It recognizes the sticky ends.

C

Explanation:

Restriction enzymes, also known as restriction endonucleases or DNA-cutter identify the base pairs to act on by targeting and binding to the recognition site in a gene.

The recognition site in a gene/DNA consists of four to eight palindromic nucleotide sequence. It is a region that the restriction enzyme cuts during the genetic engineering process in order to produce sticky or blunt ends. A gene might contain more than one recognition site.

Restriction enzymes are usually found in prokaryotic organisms, especially bacteria where they function primarily as defense molecules against bacteriophages.

Correct option: C