Biology, 15.07.2020 01:01, Hannahdavy5434
Select the order in which the following steps should be performed to express a eukaryotic protein in bacterial cells.
A. Transform bacterial cells with the plasmid.
B. Identify a cDNA clone of the gene that encodes the protein.
C. Grow the bacterial cells to high density.
D. Add allolactose to the media.
E. Break open the bacterial cells and purify the eukaryotic protein.
F. Insert the cDNA downstream of the lac control region in a plasmid.
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