With the use of site-directed mutagenesis, hemoglobin has been prepared in which the proximal histidine residues in both alpha and the beta subunits have been replaced by glycine. The imidazole ring from the histidine residue can be replaced by adding free imidazole in solution. Would you expect this modified Hb with free imidazole to exhibit oxygen binding? Why or why not?

The modified hemoglobin with free imidazole cannot be expected to show cooperativity in oxygen binding. The movement of iron ion takes place up in the plane of heme when binding of one subunit of hemoglobin takes place with oxygen. One of the iron's and oxygen's axial ligands comprise the proximal histidine's imidazole ring.  

With the movement of iron into the hemoglobin ring, the pulling of proximal histidine takes place along with it. Therefore, when binding of oxygen takes place with one subunit, a modification also takes place in the intersubunit associations, this also comprises displacement of the alpha helix. This phenomenon plays an essential role in modifying the hemoglobin's tensed state to the relaxed state. The withdrawal or mutation of the imidazole ring from the histidine residue does not further permit the cooperative binding as it is not associated physically with the alpha-helix.  

the nitrogen cycle provides nitrogen to the ecosystem from the atmosphere, ground and oceans. nitrogen is an important component of complex molecules such as amino acids and nucleotides, which lead to the creation of proteins and dna, the building blocks of all life. plants absorb nitrogen in the form of ammonium, nitrate ion and, on rare occasions, as amino acids. animals receive nitrogen necessary for biological processes from feeding on living or dead organic matter.