Biology
Biology, 06.05.2020 01:38, FlyToTheGalaxy

In a typical Sanger sequencing reaction, the amount of dNTPs is much greater than of the ddNTPs. If equal amounts of both types of nucleotides were used in each reaction, how might that affect the outcome? Group of answer choices After gel electrophoresis and visualization of the results, no bands would be visible because the high concentration of ddNTPs would inhibit the activity of the polymerase. After gel electrophoresis and visualization of the results, mostly short fragments would be present and only sequences very close to the primer could be read. After gel electrophoresis and visualization of the results, only the purine reactions would show termination and be read from the gel. After gel electrophoresis and visualization of the results, mostly long fragments would be present and no sequence could be read close to the primer. After gel electrophoresis and visualization of the results, the sequence could be read normally. The dNTP/ddNTP ratio does not affect the products of the reaction.

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In a typical Sanger sequencing reaction, the amount of dNTPs is much greater than of the ddNTPs. If...

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