After running your purified DHFR fusion protein+Laemmli 40uL sample, along with a protein ladder you transfer protein on the gel to a nitrocellulose filter for Western Blot analysis.
a. Which of the following primary antibodies could you use to detect your fusion protein based on the fusion protein identified and process described above: Anti-Myc, Anti-FLAG, Anti-GST, Anti-His, Anti-DHFR, Anti-Tubulin? List all that apply

Explanation:

The DHFR protein was fused with His tag. The DHFR fusion protein was purified and run in the SDS-PAGE for the western blotting. After the completion of gel run, the protein was transferred into the nitrocellulose membrane.

a. For the detection of fusion protein two types of primary antibody can be used. These are Anti-his and Anti-DHFR.

Anti-his antibody can be used as the protein was tagged with histidine, and anti-DHFR antibody can also be used.

yes? sounds right, but you didn’t ask a question.