A bacterial operon is responsible for the production of biosynthetic enzymes needed to make the hypothetical amino acid tisophane (tis). The operon is regulated by a separate gene, R. The deletion of R causes the loss of enzyme synthesis. In the wild-type condition, when tis is present, no enzymes are made; in the absence of tis, the enzymes are made. Mutations in the operator gene (O−) result in repression regardless of the presence of tis.
Required:
Is the operon under positive or negative control? Propose a model for:
a. repression of the genes in the presence of tis in wild-type cells.
b. the mutations.
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