The best evidence that prokaryotic genes cannot contain introns is that, Group of answer choices the introns are cut out during binary fission. integrated viral DNA is not recognized for transcription. bacterial proteins are very short and not subject to mutation. translation sometimes begins before transcription has been completed because prokaryotes do not have a nucleus. due to the short time the bacterium is around as a single organism, a 5' cap and poly (A) tail are added immediately for translation to take place.