Chromatography columns have a limited protein binding capacity. During lab #4 you should not have "overloaded" the Ni+2 Agarose column. Yet you may have noticed that the W2 fraction fluoresced slightly. If you see a band in the Western blot W2 lane, what does this data say about the physical protein structure of rGFP in that lane? Give a possible MW for this band based upon the data (show your work).