You work in a laboratory that studies the molecular biology of tribbles. [Although tribbles are an alien life form, assume here that the molecular biology of tribbles is identical to that of eukaryotes on Earth.]Your lab has a genomic library of tribble DNA, as well as a cDNA library made with mRNA extracted from whole tribbles. The lab also has a collection of live tribbles that can be used to isolate RNA or DNA, and a supply of fixed tribbles that can be stained for gene expression. Your advisor provides you with a cloned 100 bp DNA fragment that represents part of the protein-coding region of a tribble gene. Using the tools described in the previous paragraph and the molecular biology techniques we have discussed in class, how would you accomplish each of the following aims? Note: try to come up with the simplest and modest direct approach that will give you the desired information. A. Determine the amino acid sequence of the complete protein produced by that gene. B. Determine whether or not the gene contains introns. C. Determine whether the RNA produced by that gene experiences alternative splicing. D. Determine the length of the mature mRNA(s) produced by the gene. This includes the UTRs and the poly-A tail. E. Determine which cells in the tribble body do and do not express mRNA from this gene. F. You discover a blood stain in the lab, and you want to determine whether it is human blood or tribble blood. How can you do this using the molecular biology tools described above?
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You work in a laboratory that studies the molecular biology of tribbles. [Although tribbles are an a...
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