You are using genetic engineering to design a protein that needs to be synthesized in the cytoplasm and then localized in the nucleus of the cell. To accomplish this, one could add You are using genetic engineering to design a protein that needs to be synthesized in the cytoplasm and then localized in the nucleus of the cell. To accomplish this, one could add a nuclear localization signal into the DNA that will be present in the mature protein. the compound during mitosis, at a time when the nuclear membrane is dispersed. a nucleotide or few nucleotides to the protein. a series of specific polysaccharides to the carboxyterminal end of the protein. all of the above