What is the process of structural determination? What are the common techniques for structural determination?

Explanation:

Structural determination is a process that use analytical technique to describe in detailed the three dimensional positional coordinates of the scattered centre in an ordered Crystal lattice.

The techniques used are;

X- Ray crystallography, it usese the X-ray diffraction pattern. The structures to be determined by this is first of undergo purification and crystallisation, it is then expose beam of X-ray, which then diffract. It provide detailed atomic information of the structure.

NMR spectroscopy is can be use to determine the structure of protein. The structure to be determined is first of all purified, it is then exposed to strong magnetic field and radio waves. This method supply detailed information of protein in solution.

3D Electron microscopy is used to determined the 3D structures of bigger macromolecules. It uses beam of electron and lenses to show the image of electrons directly.

this protocol describes a detailed procedure for intracellular flow cytometry staining.