He protein catalase catalyzes the reaction
2 H 2 O 2 ( aq ) ⟶ 2 H 2 O ( l ) + O 2 ( g ) 2H2O2(aq)⟶2H2O(l)+O2(g)

and has a Michaelis–Menten constant of K M = 25 mM KM=25 mM and a turnover number of 4.0 × 10 7 s − 1 4.0×107 s−1 . The total enzyme concentration is 0.030 μM 0.030 μM and the initial substrate concentration is 5.09 μM 5.09 μM . Catalase has a single active site.

Calculate the value of max (often written as max ) for this enzyme.

max=

mM⋅s−1

Calculate the initial rate, (often written as 0 ), of this reaction.

=

mM⋅s−1

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