Which statement regarding the Polymerase Chain Reaction (PCR) is TRUE? Group of answer choices The primers that are used to start a PCR reaction must be double-stranded and cannot be complementary to the target DNA. PCR uses a DNA polymerase that functions best at cold temperatures. A single PCR reaction can be used to duplicate an entire genome. The amount of target DNA segments doubles with each cycle of PCR.

The amount of target DNA segments doubles with each cycle of PCR.

Explanation:

A polymerase chain reaction is an in vitro technique that is performed in the labs to replicate the DNA from a very small amount of DNA sample. The DNA sample is replicated or double in a very small amount of time that is the target sample doubles after each cycle.

The PCR technique is based on the DNA replication mechanism and thus employs the primer, A Taq DNA polymerase enzyme and the adequate amount of temperature.

Thus, the selected option is correct.