TP synthesis in glycolysis: substrate-level phosphorylationThe ATP that is generated in glycolysis is produced by substrate-level phosphorylation, a very different mechanism than the one used to produce ATP during oxidative phosphorylation. Phosphorylation reactions involve the addition of a phosphate group to another molecule. Sort the statements into the appropriate bin depending on whether or not they correctly describe some aspect of substrate-level phosphorylation in glycolysis. a. One of the substrates is a molecule derived from the breakdown of glucoseb. The enyzmes involved in ATP synthesis must be attached to a membrane to produce ATP. c. The phosphate group added to ADP to make ATP comes from free inorganic phosphate ions. d. An enzyme is required in order for the reaction to occure. A bond must be broken between an organic molecule and phosphate before ATP can form.

Correct statements sorted are about substrate level phosphorylation in glycolysis are:

a) One of the substrates is a molecule derived from the breakdown of glucose.

d) An enzyme is required in order for the reaction to occur.

e).A bond must be broken between an organic molecule and phosphate before ATP can form.

Incorrect statements found are:

b) The enyzmes involved in ATP synthesis must be attached to a membrane to produce ATP.

c). The phosphate group added to ADP to make ATP comes from free inorganic phosphate ions.

Explanation:

The substrate level phosphorylation of ADP to ATP in glycolysis occurs in cytoplasm as this is the pay-off phase in glycoloysis. Two enzymes used in the phosphorylation are:

Phospho glycerate kinase enzyme acts on molecule derived from glucose i.e 1.3 bis phosphoglycerate.

Pyruvate kinase converting phosphoenol pyruvate to pyruvate and ATP.

Glucose is a compound having 6 carbon, in the glycolysis cycle it forms 2 three carbon compound called pyruvate is formed. In total 4 ATPs are generated but 2 get used up in the breaking of glucose.