Choose the answer that has these events of protein synthesis in the proper sequence 1. The first tRNA with Met binds to the P site 2. A peptide bond forms between the new amino acid and a polypeptide chain 3. tRNA comes in to the A site with a new aa, 4. A small ribosomal subunit binds with mRNA 5. tRNA in the P site leaves and the tRNA from the A site translocates to the P site

4. A small ribosomal subunit binds with mRNA;

1. The first tRNA with Met binds to the P site;

3. tRNA comes in to the A site with a new aa;

2. A peptide bond forms between the new amino acid and a polypeptide chain;

5. tRNA in the P site leaves and the tRNA from the A site translocates to the P site.

Explanation:

The first step in translation i.e. protein synthesis is attachment of small ribosomal subunit i.e. 30S subunit with mRNA. Soon after that a tRNA charged with Met binds to the P site of 30 S ribosomal subunit. To this complex further 50S ribosomal subunit binds. Together all these components form '70 S initiation complex". Since this complex already has one amino acid (tRNA with Met) at the P site, in order to create peptide chain another tRNA charged with amino acid must enter the complex. The upcoming tRNA enters the initiation complex at A site. Next, due to the peptidyl transferase activity of large ribosomal subunit i.e. 50S subunit a peptide bond is formed between the amino acid at P site and amino acid at A site. Now since peptide bond has already been formed tRNA at P site is useless so it has to be expelled from the initiation complex via exit site. As soon as this tRNA is expelled, the tRNA which was at A site translocates into P site so that a new tRNA charged with another amino acid could enter the A site for further elongation of peptide chain.

affects the water bodies: excessive use of fertilizers causes eutrophication. the fertilizers which are used on plants affect the quality of land on which it is used, further causing erosion of soil. the fertilizers used on plants contain harmful chemical fumes which affect the environment.