Biology, 20.03.2020 11:04, janiyanmartin07
A student interested in the regulation of facultative heterochromatin needs to optimize the experimental conditions of her chromatin immunoprecipitation (ChIP) assay using heart tissue. For this, she crosslinks mouse heart tissue with 1% formaldehyde for either 10 or 30 minutes. The student then performs ChIP with the RING1B and SUZ12 antibodies. RING1B and SUZ12 belong to the Polycomb Repressor complex 1 (PRC1), which maintains genes silenced in specific tissues. The enriched DNA is quantified by real-time PCR using primers to the indicated genes. The amount of immunoprecipitated DNA in each sample is represented as normalized signal to the negative locus GAPDH, which is equal to 1.A. Why does the student consider GAPDH a negative locus, i. e. where RING1B and SUZ12 do not bind?
B. What is the transcriptional status of the HoxD10 and HoxA1 genes in the heart? In one sentence explain your reasoning.
C. If you were this student, which fixation condition (10 or 30 min) would you use? In one sentence explain your reasoning.
Answers: 2
Biology, 22.06.2019 04:30, seannalove6168
Long term exposure to waves can cause sunburns and skin cancer.
Answers: 1
Biology, 22.06.2019 10:30, jaydahh4059
How do the evolutionary chart and the video support the claim that all living things are made up of cells?
Answers: 3
Biology, 22.06.2019 15:10, cupcake3103670
Brian made this table to organize his notes on changes of state change of state energy change melting gain freezing lose deposition gain condensation lose
Answers: 1
A student interested in the regulation of facultative heterochromatin needs to optimize the experime...
Mathematics, 14.05.2021 03:30
Biology, 14.05.2021 03:30
Mathematics, 14.05.2021 03:30
Mathematics, 14.05.2021 03:30
Mathematics, 14.05.2021 03:40