He Michaelis–Menten equation is an expression of the relationship between the initial velocity V 0 of an enzymatic reaction and substrate concentration [ S ] . There are three conditions that are useful for simplifying the Michaelis–Menten equation to an expression from which the effect of [ S ] on the rate can be more readily determined. Match the condition (e. g., [ S ] = K m ) with the statement or statements that describe it. Doubling [S] will almost double the rate.
Half of the active sites are occupied by substrate.
About 90% of the active sites are occupied by substrate.
Doubling [S] will have little effect on the rate.
Less than 10% of the active sites are occupied by substrate.
This condition will result in the highest rate.
The three conditions to choose/match from are [S]=0.1 Km, [S]=Km, and [S]=10 Km