What are the 1st and 2nd line of defence of plant against pathogens?

These may be:

physical defenses  (1st line)chemical defenses (2nd line)

Explanation:

Plants employ several methods in order to defend themselves from pathogens. These may be physical defenses or chemical ones. Several have adaptations to their surfaces that prevent the entry of pathogens, called physical barriers such as

thick waxy cuticlesthorns, which are modified plant branchesspines, which are modified plant leavesgall production at infected sites to prevent pathogenic spread

Chemical defenses include the production or secretion of:

defensins antimicrobials such as tannins or resinsgum at areas facing pathogenic attacks

The answer will be d

Telophase colchicine is a chemical obtained from plants that prevents the assembly of spindle fibers