The A260/A280 ratio method estimates protein by measuring the absorbance maximum at 280nm caused by the amino acids, , and . Since also absorbs in the UV range, we can correct for this contaminate by measuring the absorbance maximum at and using the following equation:

Explanation:

The A260/A280 ratio method estimates protein purity by measuring the absorbance maximum at 280nm caused by the amino acids aromatic rings, peptide bonds, primary structure, secondary structure, and tertiary structure. Since nucleic acid also absorbs in the UV range, we can correct for this contaminate by measuring the absorbance maximum at 260nm and using the following equation.

hydroxyl -oh

alcohols, always polar, can form hydrogen bonds with h2o molecules

carboxyl -cooh  

cargoxylic acids, has acidic properties which is a source of hydrogen ions, found in cells in ionized for with a charge of oh-1

carbonyl > co

ketone if within skeleton, aldehyde if group is at the end of skeleton, k & a may be structural isomers, it determines 2 sugars (ketose & aldose)

amino -nh2

amines, acts as a base, it can pick up h+ ions from surrounding solution, ionized with charge of 1+

methyl ch2

addition to rna/dna affects and gene expression

phosphate -opo3 ²⁻

organic phosphates, contributes to negative charge to, molecule that it is a part of, and potential to react with h20

sulfhydryl -sh

thiols, cross-linking stabilize the protein, and it is structure

answer: all : )

explanation: