In samples of DNA isolated from two unidentified species of bacteria, adenine makes up 32 and 17%, respectively, of the total bases. 1. What relative proportions of adenine, guanine, thymine, and cytosine would you expect to find in the two DNA samples? (hint: bacterial DNA is circular plasmid) 2. What assumptions have you made? One of these bacteria was isolated from a hot spring 64 degree C. Which DNA came from this thermophilic bacterium? What is the basis for your answer? (hint: consider bonding strength A-T or G-C)

1. Sample 1= A=32, T=32, G=18, C=18

Sample 2:

2. Chargaff rule (A=T and G=C)

3. Sample 2

Explanation:

Sample 1= Adenine percentage= 32%

According to the Chargaff rule, thymine percentage in this sample DNA would be= 32%  

Sum total of adenine and thymine in sample 1= 32 + 32 =64%

Sum total of GC content in the sample 1= 100-64= 36

Percentage of guanine bases in sample 1= 36/2=18%

Percentage of cytosine bases in sample 1= 18%

Sample 2

Adenine percentage= 17%

According to the Chargaff rule, thymine percentage in this sample DNA would be= 17%  

Sum total of adenine and thymine in sample 2=17 + 17 = 34%

Sum total of GC content in the sample 2= 100-34= 66

Percentage of guanine bases in sample 2= 66/2=33%

Percentage of cytosine bases in sample 2= 33%

2. Percentage of adenine= percentage of thymine

Percentage of guanine= percentage of cytosine

3. DNA from sample 2 is from the bacterial living in hot water spring since it has a higher percentage of GC (66%) than sample 1 (36%). The GC bases have three hydrogen bonds between them and are relatively more stable base pairs than the AT pairs each with two hydrogen bonds.

d. groundwater flows through the subsoil and deposits minerals.

look at this it will give you up to 17 answers