Calculate how much primary antibody you need to add to 5.0 ml of TBST buffer to give a 1:3333 dilution of the primary antibody (mouse anti-GFP.)

1.5 micro liter.

Explanation:

Antibodies may be defined as the substance hat are produced by the immune cells of the body against the particular antigen. The antibodies concentration is helpful to determine the infection stages.

The amount of primary antibody can be calculated by teh following formula:

N1V1 = N2V2

Here, N1 = amount of primary antibody = 1, N2  is dilution factor  1/3333 and V2 is 5 ml = 5000 micro liter.

Then V1 can be find as follows;

V1 × 1 = 1/3333 x 5000

V1 = 1.5 micro liter.

Thus, the answer is 1.5 micro liter.

step 1: nitrogen fixation- conversion of atmospheric no₂ into forms useful to plants by lightning, bacteria, and cyanobacteria.

step 2: nitrification-process of converting ammonia to nitrite or nitrate.

step 3: assimilation-plant roots absorb new form of nitrogen.

step 4: ammonification-conversion of organic nitrogen to ammonia by bacteria involved in decomposition.

step 5: denitrification-bacteria turns the decomposed matter back into n₂o, which is released into atmosphere; repeat cycle.