Estimation of Vmax and Km by Inspection Although graphical methods are available for accurate determination of the Vmax and Km of an enzyme-catalyzed reaction (see Box 6-1), sometimes these quantities can be quickly estimated by inspecting values of V0 at increasing [S]. Estimate the Vmax and Km of the enzyme-catalyzed reaction for which the following data were obtained:

S [M] vo (μM/min)
2.5 x 10^-6 28
4.0 x10^-6 40
1.0 x 10^-5 70
2.0 x 10^-5 95
4.0 x 10^-5 112
1.0 x 10^-5 128
2.0 x 10^-3 139
1.0 x 10^-2 140

Vmax: Km: