![Biology](/tpl/images/cats/biologiya.png)
Forward primer: 5'-AGTCTACTCGTAACCGGTTACC-3' Reverse primer: 5'-TAAGGCATCATGGTAACCGGTT-3' The two primers above have the melting temperatures between 55C-80C. What is the reason that these two primers will not bind effectively to the region of DNA that we want to amplify?a. The length of the two primers is between 20-30 base pairs long. The length is too short and not very specific. b. The 3'-end of the reverse primer (3'-TTGGCCAATGG---5') is complementary to the forward primer (5'---AACCGGTTACC-3') and thus reduces the ability of the primers to bind to the targeted DNA sequence. c. The 3'-end of the forward primer is complementary to the 5'-end of the reverse primer and thus reduces the ability of the primers to bind to the targeted DNA sequence. d. The melting temperature range of the primers is similar to the DNA. e. Guanine and Cytosine make up 60% of the total base pairs.
![answer](/tpl/images/cats/otvet.png)
Answers: 2
Other questions on the subject: Biology
![image](/tpl/images/cats/biologiya.png)
Biology, 21.06.2019 19:30, ooooooo828282828
The body is protected against harmful flu viruses by
Answers: 1
![image](/tpl/images/cats/biologiya.png)
Biology, 22.06.2019 05:30, ddavid9361
Which of the following produces carbon dioxide? a. plants b. animals c. decomposers d. all of the above
Answers: 1
![image](/tpl/images/cats/biologiya.png)
![image](/tpl/images/cats/biologiya.png)
Biology, 22.06.2019 16:30, JOEFRESH10
Which part of the spine is likely to cause a nerve signal to travel faster? a. backbones b. white matter c. nuclei d. gray matter
Answers: 2
Do you know the correct answer?
Forward primer: 5'-AGTCTACTCGTAACCGGTTACC-3' Reverse primer: 5'-TAAGGCATCATGGTAACCGGTT-3' The two pr...
Questions in other subjects:
![Konu](/tpl/images/cats/mat.png)
![Konu](/tpl/images/cats/ap.png)
Advanced Placement (AP), 24.11.2020 21:50
![Konu](/tpl/images/cats/mat.png)
Mathematics, 24.11.2020 21:50
![Konu](/tpl/images/cats/mat.png)
![Konu](/tpl/images/cats/fizika.png)
Physics, 24.11.2020 21:50
![Konu](/tpl/images/cats/en.png)
![Konu](/tpl/images/cats/himiya.png)
![Konu](/tpl/images/cats/mat.png)
Mathematics, 24.11.2020 21:50
![Konu](/tpl/images/cats/en.png)
![Konu](/tpl/images/cats/biologiya.png)
Biology, 24.11.2020 21:50