Forward primer: 5'-AGTCTACTCGTAACCGGTTACC-3' Reverse primer: 5'-TAAGGCATCATGGTAACCGGTT-3' The two primers above have the melting temperatures between 55C-80C. What is the reason that these two primers will not bind effectively to the region of DNA that we want to amplify?a. The length of the two primers is between 20-30 base pairs long. The length is too short and not very specific. b. The 3'-end of the reverse primer (3'-TTGGCCAATGG---5') is complementary to the forward primer (5'---AACCGGTTACC-3') and thus reduces the ability of the primers to bind to the targeted DNA sequence. c. The 3'-end of the forward primer is complementary to the 5'-end of the reverse primer and thus reduces the ability of the primers to bind to the targeted DNA sequence. d. The melting temperature range of the primers is similar to the DNA. e. Guanine and Cytosine make up 60% of the total base pairs.

Where does all the water go? according to the environmental protection agency (epa), in a typical wetland environment, 39% of the water is outflow; 46% is seepage; 7% evaporates; and 8% remains as water volume in the ecosystem (reference: united states environmental protection agency case studies report 832-r-93-005). chloride compounds as residuals from residential areas are a problem for wetlands. suppose that in a particular wetland environment the following concentrations (mg/l) of chloride compounds were found: outflow, 60.4; seepage, 73.7; remaining due to evaporation, 26.4; in the water volume, 46.8. (a) compute the weighted average of chlorine compound concentration (mg/l) for this ecological system. (round your answer to one decimal place.) mg/l (b) suppose the epa has established an average chlorine compound concentration target of no more than 58 mg/l. does this wetlands system meet the target standard for chlorine compound concentration? yes. the average chlorine compound concentration (mg/l) is too high. yes. the average chlorine compound concentration (mg/l) is lower than the target. no. the average chlorine compound concentration (mg/l) is lower than the target. no. the average chlorine compound concentration (mg/l) is too high.

What maintain homeostasis when a persons internal body temperature is 97.5°f

Below are the three main organs that make up the plant body. what is the main function of the structure that is identified as b in the picture above? it anchors the plant. it produces food. it absorbs nutrients. it supports the plan