Last Friday after dealing with the stressors of daily life, Jane was likely to in her blood stream.

Last Friday after dealing with the stressors of daily life, Jane was likely to have increased level of cortisol in her blood stream.

Explanation:

Daily life activities, responsibilities, duties, events all leads to a lot of stress among individuals. This stress affects our body, thoughts, mind, feelings and behavior.  

Cortisol is a stress hormone secreted by the adrenal glands by the triggering of adenocorticotropic hormone of the pituitary gland.

Stressors due to daily life activities and other health problems leads to cortisol awakening response and increases the level of cortisol in the blood stream in order to cope up with the increased stress.

Increased cortisol levels causes a number of health issues like anxiety, depression, headache, digestive issues, cardiac problems, sleep disturbance, cognitive impairment etc

this protocol provides a detailed procedure for nucleoprotein extraction from cultured cells and tissues in an efficient manner.