Conjugation and Gene Mapping in Bacteria - Copy
In your research on amino acid biosynthe...
Conjugation and Gene Mapping in Bacteria - Copy
In your research on amino acid biosynthesis in E. coli, you have identified three new auxotrophic mutants in a mutagenesis screen. Each mutant can grow on minimal medium only if it is supplemented with a specific amino acid, as follows:
lys2 mutants require lysine.
gly3 mutants require glycine.
phe1 mutants require phenylalanine.
As part of your research, you decide to map the locations of the three mutant loci.
Part A - Genotyping exconjugants on supplemented media
To make a rough map of the lys2, gly3, and phe1 loci, you perform an interrupted conjugation experiment between the following two strains:
Hfr lys2+ gly3+ phe1+ AmpS � F� lys2� gly3� phe1� AmpR
(Note: AmpS indicates sensitivity to ampicillin. AmpR indicates resistance to ampicillin.)
You interrupt conjugation after 1 min, 2 min, and 3 min and plate exconjugants on minimal medium containing ampicillin and various combinations of lysine, glycine, and phenylalanine. You then score the resulting plates for the presence of colonies.
Based on the results shown below, what are the possible genotypes of the colonies that grew on each medium? Answer the question by dragging one pink label to each pink target, one white label to each white target, and one blue label to each blue target. Labels may be used once, more than once, or not at all
Answers: 1
Biology, 21.06.2019 19:30, goob92
Which statement best describes a keystone species? o a. it has littlelmenetic variation. it has an extremely high population. 0 b. 0 c. it remains unaffected by all environmental changes. od. it has a strong influence on the ecosystem. 0 e. it's the first species to populate a new ecosystem.
Answers: 2
Biology, 22.06.2019 02:30, kajjumiaialome
4. rat liver is able to metabolize glucose by both the glycolytic and the pentose phosphate pathway. which of the following are properties of both the glycolytic and pentose phosphate pathway? a. nad+ is involved b. co2 is liberated c. phosphate esters are intermediates d. glyceraldehyde 3-phosphate is an intermediate e. fructose 6-phosphate is an intermediate4. rat liver is able to metabolize glucose by both the glycolytic and the pentose phosphate pathway. which of the following are properties of both the glycolytic and pentose phosphate pathway? a. nad+ is involved b. co2 is liberated c. phosphate esters are intermediates d. glyceraldehyde 3-phosphate is an intermediate e. fructose 6-phosphate is an intermediate
Answers: 3
Biology, 22.06.2019 05:10, Jakeandjenn21
7. describe two strategies that parents can use to cope with stress. 8. describe the three types of childcare available to families. 9. what can parents do to be more involved in their children's education at home and at school? 10. which factors should a parent consider when choosing a childcare option?
Answers: 1
Biology, 22.06.2019 06:00, clonetrooper099
What is the statement plant growth is affected by the color light
Answers: 2
Physics, 09.04.2021 01:00
Mathematics, 09.04.2021 01:00
Mathematics, 09.04.2021 01:00
Mathematics, 09.04.2021 01:00